sanience-d3 sanience-d3

In vitro assessment of the absorption efficiency of vitamin D3 (cholecalciferol) in oral spray form.

The aim of the study

The aim of the study was to investigate the permeability and safety in the mucus membranes of the mouth of various vitamin D3 aerosols available on the market, at various intervals and to demonstrate the buccal-sublingual absorption of the active substance cholecalciferol (vitamin D3).

Materials and methods

Location of the study: Laboratory of Bioanalytical methods and Biodosimetry of the Faculty of Biology of the University of Latvia

Lead researcher:Prof. Aleksejs Derovs


For the relative comparison among various samples of the efficiency of cholecalciferol (vitamin D3) diffusion, a vertical diffusion cell automatic system was selected, using cellulose membranes as a barrier. Time points selected: at 30 minutes and slightly later at 3 hours, as well as at 16 hours.


Image 1. Migration results for assayable samples of vitamin D3 at all time points

General conclusions

• The best result for vitamin D3 migration through cellulose membranes at all tested time points were demonstrated by the formula (see Image 1).

• The assayed compositions containing vitamin D3 do not have a significant negative effect on the viability of epithelial tissue in the mucous membranes of the mouth, which demonstrates the safety of the formulations.

*For more details about the study, see:
Prof. Aleksejs Derovs, “Dažādu D3 vitamīna formu uzsūkšanās efektivitātes analīze”, magazine “Latvijas ārsts”, October 2020 issue

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